Whole genome amplification and exome sequencing of archived schistosome miracidia

Adult schistosomes live in the blood vessels and cannot easily be sampled from humans, so archived miracidia larvae hatched from eggs expelled in feces or urine are commonly used for population genetic studies. Large collections of archived miracidia on FTA cards are now available through the Schistosomiasis Collection at the Natural History Museum (SCAN). Here we describe protocols for whole genome amplification of Schistosoma mansoni and Schistosome haematobium miracidia from these cards, as well as real time PCR quantification of amplified schistosome DNA. We used microgram quantities of DNA obtained for exome capture and sequencing of single miracidia, generating dense polymorphism data across the exome. These methods will facilitate the transition from population genetics, using limited numbers of markers to population genomics using genome-wide marker information, maximising the value of collections such as SCAN.

Citation

Le Clec'h, W., Chevalier, F., McDew-White, M., Allan, F., Webster, B., Gouvras, A., . . . Anderson, T. (2018). Whole genome amplification and exome sequencing of archived schistosome miracidia. Parasitology, 145(13), 1739-1747. doi:10.1017/S0031182018000811

Type

Journal Article

Item Description

This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike licence (http://creativecommons.org/licenses/by-nc-sa/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the same Creative Commons licence is included and the original work is properly cited. The written permission of Cambridge University Press must be obtained for commercial re-use. The attached file is the published version of this article.
NHM Repository

ISSN

0031-1820

EISSN

1469-8161